GFP-tagged balancer chromosomes for Drosophila melanogaster

GFP-tagged balancer chromosomes for Drosophila melanogaster

We constructed green fluorescent protein (GFP)-expressing balancer chromosomes for each of the three major chromosomes of Drosophila melanogaster. Expression of GFP in these chromosomes is driven indirectly by a Kruppel (Kr) promoter, via the yeast GAL4-UAS regulatory system. GFP fluorescence can be seen in embryos as early as the germ band extension stage, and can also be seen in larvae, pupae...

Rare recombination events generate sequence diversity among balancer chromosomes in Drosophila melanogaster.

Multiply inverted balancer chromosomes that suppress exchange with their homologs are an essential part of the Drosophila melanogaster genetic toolkit. Despite their widespread use, the organization of balancer chromosomes has not been characterized at the molecular level, and the degree of sequence variation among copies of balancer chromosomes is unknown. To map inversion breakpoints and stud...

Tubby-tagged balancers for the Drosophila X and second chromosomes.

We generated FM7a and CyO balancer chromosomes bearing a Tubby1 (Tb1) dominant transgene. Flies heterozygous for these FM7a and CyO derivatives exhibit a phenotype undistinguishable from that elicited by the Tb1 mutation associated with the TM6B balancer. We tested two of these Tb-bearing balancers (FM7-TbA and CyO-TbA) for more than 30 generations and found that the Tb1 transgene they carry is...

Distributive Pairing of Compound Chromosomes in Females of Drosophila Melanogaster.

HE relation between crossing-over and disjunction in the Drosophila female Thas been studied for many years. ANDERSON (1929), DOBZHANSKY (1932, 1933), BROWN (1940), PIPKIN ( 1940) and others clearly demonstrated that crossing-over has a strong influence on disjunction in translocation heterozygotes. The reciprocal products of an exchange tend to pass to opposite poles of the first meiotic divis...

The units of DNA replication in Drosophila melanogaster chromosomes.